Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy
نویسندگان
چکیده
منابع مشابه
Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy.
We used fluorescence microscopy to determine how polymerization of Mg-ADP-actin depends on the concentration of phosphate. From the dependence of the elongation rate on the actin concentration and direct observations of depolymerizing filaments, we measured the polymerization rate constants of ADP-actin and ADP-P(i)-actin. Saturating phosphate reduces the critical concentration for polymerizati...
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Using hexokinase, glucose, and ATP to vary reversibly the concentrations of ADP and ATP in solution and bound to Acanthamoeba actin, I measured the relative critical concentrations and elongation rate constants for ATP-actin and ADP-actin in 50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 0.1 mM nucleotide, 0.1 mM CaCl2, 10 mM imidazole, pH 7. By both steady-state and elongation rate methods, the critical co...
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The involvement of interactions between ATP-actin and ADP-actin in actin polymerization has been studied. It has been found that ATP-actin and ADP-actin can copolymerize and that the rate of nucleation is enhanced when both ATP-actin and ADP-actin are present in solution. The fact that the heterologous interaction between ATP-actin (T) and ADP-actin (D) is stronger than either of the homologous...
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umber of clostridial bacteria produce toxins which ADP-ribosylate monomeric actin. These include botulinum C2 toxin (2), Clostridiumperfringens iota toxin (23, 26), Clostridium spiroforme toxin (20, 27), and an ADP-ribosyltransferase produced by Clostridium difficile (21). The toxins have turned out to be valuable tools for investigating the actin cytoskeleton since ADP-ribosylation of actin bl...
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This minireview is dedicated to the memory of Henryk Eisenberg and honors his major contributions to many areas of biophysics and to the analysis of macromolecular states and interactions in particular. This work reviews the ATP and ADP states of a ubiquitous protein, actins, and considers the present evidence for and against unique, nucleotide-dependent conformations of this protein. The effec...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2007
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0702510104